Genopure plasmid midi kit roche manual
GENOPURE PLASMID MIDI KIT ROCHE MANUAL >> READ ONLINE
After using this convenient kit, the purified plasmid DNA is suitable for downstream application such as transfection, in vitro transcription and translation, and all enzymatic modification. Specification: Sample Size: up to 60 ml of bacteria for high-copy number plasmid up to 120 ml of bacteria for low-copy Roche genopure plasmid midi kit 1 kit (for up to 20 preparations). Код: Германия • Поставщик: Roche. 154 DNA Isolation Kit for Cells and Tissues 230 Real-Time PCR Instruments. 143. Genopure Buffer Set for Low-Copy Number Plasmids . 150. Chapter 4Solution-based Isolation. 2. Plasmid Midi Kit (Roche, catalog number: 03143414001) (e.g. Genopure Plasmid Midi Kit). A. DNA preparation 1. Prepare plasmids using commercial kits (e.g. Genopure Plasmid Midi Kit). 2. Dilute DNA in sterile water to final concentration of 1 ?g/?l. The E.Z.N.A.® Plasmid DNA Midi and Maxi Kits combine the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiBind® DNA columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be Plasmid DNA preparations were obtained using Genopure Plasmid Midi kit (Roche) following kit instructions. Digoxigenin-UTP-labeled antisense probes for in situ hybridization (ISH) were synthesized and purified using Super Script kit (Roche) following the manufacturer instructions. Instruction Manual. 11. - Note - Integrity of kit components is guaranteed for up to one year from date of purchase. 2 The ZymoPURE™ Elution Buffer contains 10 mM Tris-HCl, pH 8.5 & 0.1 mM EDTA. If required, pure water can also be used to elute the DNA. Plasmids were extracted from Escherichia coli using Genopure Plasmid Midi Kit (Roche), according to the instructions of the manufacturer. Plasmid DNA was precipitated with ethanol and then dissolved in water. Genorise Pure Plasmid Mini Kit Cat No. DNA binding columns Colletion Tubes Buffer GL ?Optional steps? Buffer G1 Buffer G2 Buffer A. Selection and Preparation of Plasmids and E. coli Strains Plasmid DNA can be purified from overnight cultures of E. coli with the QIAGEN Plasmid Mini, Midi, and Maxi Kits (EN). When low-copy-number plasmids containing the pMB1 or ColE1 origin of replication are prepared using QIAGEN Plasmid Purification Kits, plasmid DNA yields can be improved by adding chloramphenicol to the
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